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Animal Total RNA Isolation Kit Total RNA Extraction and Purification Kits for Animal Tissues & Cell
Kits Descriptions
50 Preps, 200 Preps
This kit uses the spin column and formula developed by our company, which can extract high-purity and high-quality total RNA from various animal tissues with high efficiency.It provides an efficient DNA-Cleaning Column, which can easily separate and adsorb genomic DNA from the supernatant and tissue lysate, simple and time-saving; RNA-only Column can efficiently bind RNA and can be processed simultaneously with a unique formula Lots of samples.
The whole system is RNase-Free, so that the extracted RNA is not degraded; Buffer RW1, Buffer RW2 buffer washing system, so that the obtained RNA is free of protein, DNA, ion, and organic compound pollution.
Kit components
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Animal Total RNA Isolation Kit |
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| Kit components | RE-03011 | RE-03014 |
| 50 T | 200 T | |
| Buffer RL1* | 25ml | 100ml |
| Buffer RL2 | 15ml | 60ml |
| Buffer RW1* | 25ml | 100ml |
| Buffer RW2 | 24ml | 96ml |
| RNase-Free ddH2O | 10ml | 40ml |
| RNA-only Column | 50 | 200 |
| DNA-Cleaning Column | 50 | 200 |
| Instruction Manual | 1 piece | 1 piece |
Product information
| Format | Spin column | Purification component | column, reagent |
| Flux | 1-24 samples | Time per prep | ~30 min (24 samples) |
| Centrifuge | Desk centrifuge | Pyrolysis separation | Centrifugal separation |
| Sample | Animal tissue; cell | Samples amount | Tissue:10-20 mg; Cell:(1-5)×106 |
| Elution volume | 50-200 μL | Maximum loading volume | 850 μL |
Features&advantages
■ No need to worry about RNA degradation; the whole system is RNase-Free
■ Effectively remove DNA-using DNA-Cleaning Column
■ Remove DNA without adding DNase
■ Simple-all operations are completed at room temperature
■ Fast -operation can be completed in 30 minutes
■ Safe-no organic reagent required
■ High purity -OD260/280≈1.8-2.1
Kit application
It is suitable for the extraction and purification of total RNA from a variety of fresh or frozen animal tissues or cultured cells.
Product parameters
■ Downstream applications: first-strand cDNA synthesis, RT-PCR, molecular cloning, Northern Blot, etc.
■ Samples: animal tissues, cultured cells
■ Dosage: Tissues 10-20mg, Cells(2-5)×106
■ Maximum DNA binding capacity of purification column: 80 μg
■ Elution volume: 50-200 μl
Work flow
Diagram
Animal Total RNA Isolation Kit treated 20mg
Fresh mouse samples, take 5% purified total RNA 1% agar
Glycogel electrophoresis
1: Spleen 2: Kidney
3: Liver 4: Heart
Storage and shelf life
The kit can be stored for 24 months at room temperature (15–25 ℃) or 2–8 ℃ for longer time. Buffer RL1 can be stored at 4 ℃ for 1 month after adding β- mercaptoethanol (optional).
Cited articles
1. IF:18.808: Zheng, Q., Qin, F., Luo, R., et al. mRNA-Loaded Lipid-Like Nanoparticles for Liver Base Editing Via the Optimization of Central Composite Design. Adv. Funct. Mater. 2021, 31, 2011068.doi:10.1002/adfm.202011068.
2.IF:18.187:He X, Hong W, Yang J, et al. Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine. Signal Transduct Target Ther. 2021 Jul 14;6(1):270. doi: 10.1038/s41392-021-00688-z.
3.IF:17.97 
ai Z, Liu H, Liao J, et al. N7-Methylguanosine tRNA modification enhances oncogenic mRNA translation and promotes intrahepatic cholangiocarcinoma progression. Mol Cell. 2021 Jul 29:S1097-2765(21)00555-4. doi: 10.1016/j.molcel.2021.07.003.
4.IF:9.225:Cao X, Shu Y, Chen Y, et al. Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cell Mol Gastroenterol Hepatol. 2021;12(2):633-651. doi: 10.1016/j.jcmgh.2021.04.001.
RNA isoaltion kits for other sample sources are available:
Cell, plant, viral, blood, etc.
WHY CHOOSE US
Since its establishment, our factory has been developing first world class products with adhering the principle
of quality first. Our products have gained excellent reputation in the industry and valuabletrusty among new and old customers..